| protein, cleavage semiSyntaxThis parameter is a text string that may be yes or no. Notes
 DescriptionIn real experiments, there are often contaminating proteolytic enzymes which will act on proteins in a difficult to predict manner. Therefore, the idea of a "semi-tryptic" digest was developed to attempt to compensate for this additional cleavage activity. This cleavage type assumes that one of the cleavage sites is tryptic ([KR]|{P}), but the other site may be at any residue. For example, if an expected tryptic peptide has a sequence of TFGQVVAR, then the semi-tryptic peptides associated with this sequence would be: 
TFGQVVAR
 FGQVVAR
  GQVVAR
   QVVAR
    VVAR
     VAR
      AR
TFGQVVA
TFGQVV
TFGQV
TFGQ
TFG
TF
Tandem has broadened this concept to include any cleavage site specification, except for cleavage at any site ([X]|[X]), which doesn't require any additional type of cleavage. see also: protein, cleavage site | refine, cleavage semi X! TANDEM API description project |